Place a clean extraction tube in the designated area of the workstation. Add 10 drops of extraction buffer (approx. 560 μL) into the extraction tube.
Put the specimen swab into the extraction tube and vigorously mix the solution by rotating the swab forcefully against the side of the tube at least 10 times (while submerged). Best results are obtained when the specimen is vigorously mixed in the solution.
Allow the swab to soak in the reaction buffer for 1 minute prior to the next step. Squeeze out as much liquid as possible from the swab by pinching the slide of the flexible extraction tube as the swab is removed. At least 1/2 of the reaction buffer solution must remain in the tube for adequate capillary migration to occur.
Discard the swab in a suitable bio-hazardous waste container, then fit the extraction tube tip onto the extraction tube.
Remove the test cassette from its sealed pouch, and place it on a clean and level surface. To obtain the best result, the assay should be performed within one hour.
Add 3 drops of extracted sample (approx. 100 μL) from the extraction tube to the specimen well(s) on the test cassette. Please note: avoid trapping air bubbles in the specimen well(s) and do not drop any solution in the observation window.
Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret the results after 20 minutes.